Coding

Part:BBa_K2719001:Experience

Designed by: Karla Soto Blas   Group: iGEM18_TecCEM   (2018-08-08)


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Applications of BBa_K2719001

To confirm the presence of Tenascin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 1)

T--TecCEM--OPTCDColony.png

Figure 1. Colonies transformed with Tenascin 5 Domain V



To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid extraction (Figure 2) and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of GST. (Figure 3)

T--TecCEM--OPTCDEx.png

Figure 2. Tenascin 5 Domain 085% Agarose Gel with GelRed: MW) 1Kb plus from NEB; 6) Tenascin 5 Domain (BBa_K2719001)

T--TecCEM--OPTCDRES.png

Figure 3. Tenascin 5 Domain V 0.85% agarose gel with GelRed: MW) 1KB plus from NEB; 4) Tenascin 5 Domain V (BBa_K2719001)

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